J. Sci. Agri. Technol. (2020) Vol. 1 (2): 7-13
Pueraria mirifica (PM), also known as Kwao Krua Khaw, has been used as an herbal plant for many decades in the Northern part of Thailand. The root of PM contains estrogen-like compounds that are less soluble in water resulted in low bioavailability in an aquatic animal application. To increase solubility of the compounds, six different polarity solvents such as hexane, benzene ethyl acetate, acetone, ethanol, and deionized distilled water were employed for extraction. It found that deionized distilled water exhibited the highest extraction percentage yield. However, a high amount of phenolic compounds and flavonoids were obtained in ethyl acetate fraction (EAF). In addition, the results of scavenging radical assay using DPPH IC50 showed the highest activity of EAF due to the high content of total phenolic content and flavonoids content. Moreover, EAF, significantly exhibited the highest proliferation activity against MCF-7. Therefore, EAF was selected for developing polymeric micelles. The HPbCD, Pluronic F-68, and Pluronic F-127 were employed with Tween80 to prepare suitable nanoparticles. The proper ratio of EAF-loaded Pluronic F-127 (EAF-F127) product in nanosize range elicited zeta potential value of nearly zero mV and exhibited the transparent aqueous dispersions in DI water. The results promised further in vivo studies.
Keywords: Pueraria mirifica, antioxidant, MCF-7, polymeric micelles, nanoparticles
Received: September 26, 2020. Revised: November 1, 2020. Accepted: November11, 2020.